Production and Analysis of High Molecular Weight Genomic DNA for NGS Pipelines Using Agilent DNA Extraction Kit (p/n 200600) Authors

The Agilent DNA Extraction Kit (p/n 200600) was compared to standard methods such as beadbeating and enzyme treatment for preparation of genomic DNA from the prokaryote Listeria monocytogenes. Using this extraction kit, with modifications, to lyse the bacteria and isolate high molecular weight DNA reproducibly yielded high quality DNA suitable for further applications such as polymerase chain reactions to produce amplicons, or for next-generation DNA sequencing. The quality of the high molecular weight DNA, and the comparison of extraction methods, was shown on the Agilent 2200 TapeStation with the Agilent Genomic DNA ScreenTape (p/n 5067-5365) and Agilent Genomic DNA Reagents (p/n 5067-5366).